Turn on equipment
	Sheath is MilliQ water
File->System startup program
Load 2mL ddH2O, takes around 10 mins
Run QC beads
	QC standarization
		Flowtube. 3 drops of QC in 1 mL
		Tick lot number = if not then go to beckman coulter and download target value
		Setting->library import
	event per sec >100
	QC report 
		Channel gain 20% difference
		peak CV<5
	File-> close QC
New experiment
	Setting set channel - label
	Plot FSC, SDC
	Green vs count, red vs count, FSC vs SSC, green vs red
	Statistic percent gate= percent parent 
		right click- statistic setting- export csv file
		population hierarchy
		print PDF
	Run aways running
	Record conditional -> define events (10k)
		or time
		or volume
	Boost- manual 
Acquisition setting
	Gain 1-3000 (non linear below 10)
		Recommend
	Threshold
		FSC
	Width
QC
	Event rate
	aborts
	events to display
Sample flow rate
	slow-medium
	fluorescent BG 10^2 to 10^4 is ok
Troubleshooting
	Standby- Prime and deep clean
	Cytometer-daily clean